Transmission efficiency of the AP-variant 1 strain of Anaplasma phagocytophila
Date of Original Version
Nymphal Ixodes scapularis ticks were collected from several sites in Rhode Island. DNA was extracted from a subset of these ticks, and PCR and DNA sequencing of the 16S rRNA gene were used to determine the ratio of Anaplasma phagocytophila-human agent (AP-ha) to a genetic variant not associated with human disease (AP-Variant 1). The remaining ticks were allowed to feed to repletion on either white-footed (Peromyscus leucopus) or DBA/2 (Mus musculus) mice. The engorged ticks, and blood samples drawn from each mouse at one-week intervals, were evaluated by PCR and DNA sequencing for the presence of AP-ha and Variant 1. Although a high percentage of the infecting ticks harbored AP-Variant 1, only AP-ha was amplified from the mouse blood samples. Because the A. phagocytophila variant did not establish an infection either in the natural reservoir of AP-ha, the white-footed mouse, or in a common research laboratory mouse (DBA/2), AP-Variant 1 may have an alternative natural reservoir, possibly the white-tailed deer.
Publication Title, e.g., Journal
Annals of the New York Academy of Sciences
Massung, Robert F., Thomas N. Mather, Rachael A. Priestley, and Michael L. Levin. "Transmission efficiency of the AP-variant 1 strain of Anaplasma phagocytophila." Annals of the New York Academy of Sciences 990, (2003). doi: 10.1111/j.1749-6632.2003.tb07340.x.