Date of Original Version
Plant Sciences and Entomology
Powassan virus lineage 2 (deer tick virus) is an emergent threat to American public health, causing severe neurologic disease. Its life cycle in nature remains poorly understood. We use a host-specific retrotransposon-targeted real time PCR assay to test the hypothesis that white-footed mice, considered the main eastern U.S. reservoir of the coinfecting agent of Lyme disease, is the reservoir for deer tick virus. Of 20 virus-infected host-seeking nymphal black-legged ticks 65% fed on shrews and none on mice. The proportion of ticks feeding on shrews at a site is positively associated with prevalence of viral infection, but not the Lyme disease agent. Viral RNA is detected in the brain of one shrew. We conclude that shrews are a likely reservoir host for deer tick virus and that host bloodmeal analysis can provide direct evidence to incriminate reservoir hosts, thereby promoting our understanding of the ecology of tick-borne infections.
Publication Title, e.g., Journal
Goethert, H.K., Mather, T.N., Johnson, R.W. et al. Incrimination of shrews as a reservoir for Powassan virus. Commun Biol 4, 1319 (2021). https://doi.org/10.1038/s42003-021-02828-1
Available at: https://doi.org/10.1038/s42003-021-02828-1
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