Time optimization of heterogeneous immunoassay using PDMS microfluidic chip for quantitative detection of a model analyte

Document Type

Conference Proceeding

Date of Original Version



Heterogeneous immunoassay (HI) has been recently used for on-chip biomolecule detection by many researchers due to its high specificity and precision. However, the protocols that have been published are too time-consuming. For HI based immunosensor to be attractive they should have short analysis times. In our work, a polydimethylsiloxane (PDMS) microfluidic device was fabricated to quantify a model analyte (bovine IgG) and used for optimizing the time required for each process. The results show that the incubation time of one single process could be reduced from typical 1 hour to 5 minutes without loss of performance. Our studies reveal that microfluidic devices are only effective in reducing incubation times for diffusion limited processes but not for interaction or adsorption limited process. Copyright © 2008 by ASME.

Publication Title

Proceedings of the 6th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM2008