A blocking-free microfluidic fluorescence heterogeneous immunoassay for quantitative detection of human C-reactive protein
Date of Original Version
In this article, a rapid, sensitive, and disposable microfluidic immunosensor is presented for point-of-care testing and clinical diagnostics. For the first time, a heterogeneous immunoassay without blocking process is achieved by using protein A functionalized polydimethylsiloxane microchannels. C-reactive protein (CRP), a biomarker for inflammation and cardiovascular disease risk assessment, is selected as a model analyte to demonstrate the sensitivity of this blocking-free microfluidic heterogeneous immunoassay. A four parameter logistic function is used to model and assess the data. The limit of detection obtained is 0.36 (μg/mL, which is lower than the cut-off value for clinical diagnosis. The overall assay is completed in 23 min. The assay procedure can be modified for detection of other disease biomarkers or virulent pathogens by using different capture and detection antibodies. Copyright © 2009 by ASME.
ASME International Mechanical Engineering Congress and Exposition, Proceedings
Li, Peng, Radha Narayanan, Alex J. Sherry, Jairo A. Cortes, Andrew Marchesseault, Constantine Anagnostopoulos, and Mohammad Faghri. "A blocking-free microfluidic fluorescence heterogeneous immunoassay for quantitative detection of human C-reactive protein." ASME International Mechanical Engineering Congress and Exposition, Proceedings 12, PART A (2010): 439-443. doi:10.1115/IMECE2009-10904.