A paper based lateral flow device with fluid actuated valves to detect sepsis

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This study focuses on the development of a new paper based microfluidic device for the detection of Inter alpha Inhibitor Proteins (IαIP). The key development is on the application of fluidic actuated valves embedded in the paper that allow for sequential manipulation of the sample fluid and multiple reagents in a controlled manner to perform an ELISA protocol on an autonomous paper-based platform. Recent studies proved that the concentration of IαIP is related to the mortality rate of patients who suffer from sepsis. The developed device is able to determine the concentration of IαIP where a low concentration is an indication of sepsis. The microfluidic device consists of paper on which the channel geometries are printed with wax ink. Different layers of the device are stacked on top of one another and held together with double sided tape. After applying the reagents, the device produces the results autonomously in the form of a colored dot. The intensity of the dot is correlated to the concentration of the target analyte IαIP. After optimizing the reagents with regards to reproducibility and optimal signal to noise ratio and sensitivity, a standard curve in the range of 1600-10 ng/ml is produced that links the intensity of color dot to the concentration of IαIP. The limit of detection was estimated to be 25 ng ml-1 which is comparable with the results obtained by conventional ELISA.

Publication Title

Biointerface Research in Applied Chemistry





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