Differential expression of cyclic-AMP responsive element modulator (CREM) mRNA isoforms during testicular development of boars
Date of Original Version
The cyclic-AMP responsive element modulator (CREM) transcription factor, a key regulator of spermatogenesis, can activate or repress gene expression by differential expression of alternatively spliced mRNA isoforms. The objective of this study was to report the sequences of the predominant CREM mRNA isoforms with leader exons B, θ1, θ2, in addition to the relative expression of these transcripts, in immature and adult boar testes. Activator CREM isoforms τγ (containing the B exon), θ1τγ and θ2τγ, were expressed in the adult boar testes, but not in immature testes. The CREM θ2τγ isoform had the highest expression levels in the adult boar testes compared to the τγ and θ1τγ isoforms (θ2τγ = 4.94 ± 1.49 in contrast to τγ = 1.66 ± 0.79 and θ1τγ = 2.32 ± 0.72; mean ± SD; P < 0.01). Interestingly, the predominant CREM mRNA isoforms in adult boar testes contained the C and γ exons; these exons are not present in human and rodent CREM mRNA, demonstrating species-specific expression. In contrast to the adult CREM isoforms, only CREM mRNA isoforms with the B exon (τ1 and α) were amplified in the 21 d (immature) boar testes. Further understanding of CREM mRNA isoform expression and regulation during boar spermatogenesis could contribute to our knowledge of spermatogenic efficiency in this species. © 2012 Elsevier Inc..
Publication Title, e.g., Journal
Sartini, B. L., M. Eerhart, and S. Green. "Differential expression of cyclic-AMP responsive element modulator (CREM) mRNA isoforms during testicular development of boars." Theriogenology 77, 5 (2012). doi: 10.1016/j.theriogenology.2011.09.013.