Storage conditions for serum deslorelin
Date of Original Version
The stability of a luteinizing hormone-releasing hormone (LHRH) analog in rat serum was studied under reproduced experimental analysis conditions. Serum samples of deslorelin [D-Trp6, Des-Gly, NH10/2] LHRH ethylamide were exposed to multiple freeze-thaw cycles to determine the maximum number of cycles the serum sample can be exposed to without producing any quantitative changes in radioimmunoassay (RIA) measurements of deslorelin. A significant cycle effect was observed after completion of the sixth cycle. Serum samples were also stored at standardized - 50°C conditions for variable periods of time to determine the effects of acute and chronic storage time on deslorelin stability. Matched-pair t-test analysis showed no significant changes in deslorelin values as measured by RIA for a 3-week, 4-month, or 2-year storage period. The conditions in which the rat serum samples were stored prior to analytical analysis were sufficient to prevent detectable degradation of the deslorelin peptide.
Publication Title, e.g., Journal
Drug Development and Industrial Pharmacy
Underhill, L., Richard C. Rhodes, and C. T. Rhodes. "Storage conditions for serum deslorelin." Drug Development and Industrial Pharmacy 25, 9 (1999). doi: 10.1081/DDC-100102267.