Title

ULTRAVIOLET RESONANCE RAMAN SPECTRA OF ESCHERICHIA COLI WITH 222. 5-251. 0 nm PULSED LASER EXCITATION.

Document Type

Article

Date of Original Version

1-1-1988

Abstract

Resonance Raman spectra of the gram-negative organism, Escherichia coli, have been obtained with 222. 5-, 230. 6-, and 251. 0-nm excitation, and the results have been compared with those reported earlier for 242. 4-nm excitation. Major changes in bacterial spectra have been observed with changes in exciting wavelength. The origins of the major peaks in each spectrum have been explained primarily in terms of contributions of nucleic acid bases and aromatic amino acids. As an aid in making assignments, spectra of aromatic amino acids, nucleosides, and mixtures of the two have been obtained at each wavelength used to excite bacterial spectra. Background fluorescence has been observed to be negligible below 251 nm. Selective excitation of bacterial nucleic acid and protein components has been done with ease.

Publication Title, e.g., Journal

Applied Spectroscopy

Volume

42

Issue

5

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