An in vitro assay to measure phagocytosis in striped bass hybrids (Morone saxatilis × Morone chrysops)
Date of Original Version
An in vitro phagocytosis assay was developed for hybrid striped bass (Morone saxatilis×Morone chrysops), using cells collected from the peritoneal cavity of this fish. The findings indicated that: (1) 10 days following a single intraperitoneal injection (1ml) of Freund's incomplete adjuvant (FIA) was an appropriate time for collecting suitable working concentrations (5·3± 4·0×107cells ml-1) of peritoneal phagocytes (83·7±1·5% macrophages) from these hybrids held at 23°C; (2) these cells phagocytosed latex beads (polystyrene microspheres 3·12μm in diameter) after 30min of in vitro incubation at room temperature (25±1°C). The phagocytic ability and phagocytic capacity in a washed adherent layer exposure system were 67·2±2·76% and 4·14±0·35 beads phagocyte-1, respectively. These results strongly suggest that a simple methodology, including baseline data serving as guidelines, is now available for conducting in vitro phagocytosis assays in this hybrid © 2000 Academic Press.
Publication Title, e.g., Journal
Fish and Shellfish Immunology
Polonio, Roy E., Richard E. Wolke, Sharon A. Maclean, and Jay F. Sperry. "An in vitro assay to measure phagocytosis in striped bass hybrids (Morone saxatilis × Morone chrysops)." Fish and Shellfish Immunology 10, 5 (2000). doi: 10.1006/fsim.1999.0248.