Title

Vitamin K dependent formation of γ-carboxyglutamate residues in tumor microsomes

Document Type

Article

Date of Original Version

11-16-1982

Abstract

Vitamin K stimulated the incorporation of 14C into proteins when microsomes from melanoma, mammary gland, mast cell and lymphoma tumors were incubated with Na214CO3. The 14C label in the [14C] proteins was identified as [14C] γ-carboxyglutamate (Gla), which is formed by carboxylation of glutamic acid residues. Carboxylation in tumor microsomes ranged from 2 to 19% of the carboxylation in normal liver microsomes per mg of microsomal protein. Carboxylation in microsomes was completely blocked by 10 μM Warfarin. SDS-polyacrylamide gel analysis of the melanoma [14C] Gla protein(s) revealed one major peak of 14C with an apparent MW of less than 6,000. © 1982.

Publication Title, e.g., Journal

Biochemical and Biophysical Research Communications

Volume

109

Issue

1

Share

COinS