Vitamin K dependent formation of γ-carboxyglutamate residues in tumor microsomes

Authors

Steven D. Buchthal, University of Rhode Island
Cathy G. McAllister, University of Rhode Island
David C. C. Laux, University of Rhode Island
Robert G. Bell, University of Rhode Island

Document Type

Article

Date of Original Version

11-16-1982

Abstract

Vitamin K stimulated the incorporation of 14C into proteins when microsomes from melanoma, mammary gland, mast cell and lymphoma tumors were incubated with Na214CO3. The 14C label in the [14C] proteins was identified as [14C] γ-carboxyglutamate (Gla), which is formed by carboxylation of glutamic acid residues. Carboxylation in tumor microsomes ranged from 2 to 19% of the carboxylation in normal liver microsomes per mg of microsomal protein. Carboxylation in microsomes was completely blocked by 10 μM Warfarin. SDS-polyacrylamide gel analysis of the melanoma [14C] Gla protein(s) revealed one major peak of 14C with an apparent MW of less than 6,000. © 1982.

Publication Title, e.g., Journal

Biochemical and Biophysical Research Communications

Volume

109

Issue

1

Citation/Publisher Attribution

Buchthal, Steven D., Cathy G. McAllister, David C. C. Laux, and Robert G. Bell. "Vitamin K dependent formation of γ-carboxyglutamate residues in tumor microsomes." Biochemical and Biophysical Research Communications 109, 1 (1982): 55-62. doi: 10.1016/0006-291X(82)91565-0.