Role of leuX in Escherichia coli colonization of the streptomycin-treated mouse large intestine
Date of Original Version
Escherichia coli F-18, a normal human fecal isolate, is an excellent colonizer of the streptomycin-treated mouse large intestine. E. coli F-18 Col-, a derivative of E. coli F-18 that no longer makes the E. coli F-18 colicin, colonizes the mouse large intestine as well as E. coli F18 when fed alone, but is eliminated when fed together with E. coli F-18. Recently, a random bank of E. coli F-18 DNA was transformed into E. coli F-18 Col-, the resultant population was fed to streptomycin-treated mice, and the intestine was used to select the best colonizer. In this fashion, a 6.5 kb E. coli F-18 DNA fragment was isolated. This fragment was shown to enhance E. coli F-18 Col- mouse large intestinal colonizing ability and survival during stationary phase in intestinal mucus in vitro, as well as stimulate the synthesis of type-1 fimbriae. Here, we present evidence that the gene responsible for the enhanced E. coli F-18 Col- colonizing ability and survival during stationary phase in vitro is leuX. This gene encodes a rare leucine tRNA specific for the UUG codon. In addition, we show that the presence of a functional leuX gene is necessary for E. coli K-12 intestinal colonization and for survival in stationary phase. © 1994 Academic Press. All rights reserved.
Publication Title, e.g., Journal
Newman, Joseph V., Roberto Kolter, David C. C. Laux, and Paul S. Cohen. "Role of leuX in Escherichia coli colonization of the streptomycin-treated mouse large intestine." Microbial Pathogenesis 17, 5 (1994): 301-311. doi: 10.1006/mpat.1994.1076.