A Salmonella typhimurium mutant unable to utilize fatty acids and citrate is avirulent and immunogenic in mice
Date of Original Version
Salmonella typhimurium SR-11 is extremely virulent at a dose as low as 105 colony forming units (cfu) when administered perorally to BALB/c mice. Utilizing mini-transposon mutagenesis, a mutant of S. typhimurium SR-11 was isolated that was unable to utilize oleate and citrate as carbon sources. This mutant, designated S. typhimurium SR-11 Fad- (Fatty acid), was found to utilize sugars under cyalcrp control as sole carbon sources, suggesting that the mutation is not in either of these genes. In addition, SR-11 Fad- utilized pyruvate and succinate, but was unable to utilize either acetate or isocitrate as sole carbon source. In contrast to SR-11, SR-11 Fad- was found to be avirulent, i.e. BALB/c mice were completely healthy after oral infection with 109 S. typhimurium SR-11 Fad- cells. Moreover, 21 days after SR-11 Fad- infection, BALB/c mice were found to be protected against an oral challenge with 109 cells of S. typhimurium SR-11.
Publication Title, e.g., Journal
FEMS Microbiology Letters
Utley, Maryjane, David P. Franklin, Karen A. A. Krogfelt, David C. Laux, and Paul S. Cohen. "A Salmonella typhimurium mutant unable to utilize fatty acids and citrate is avirulent and immunogenic in mice." FEMS Microbiology Letters 163, 2 (1998): 129-134. doi: 10.1016/S0378-1097(98)00162-1.