In vitro assembly of nucleosomes for binding/remodeling assays
Document Type
Article
Date of Original Version
1-1-2017
Abstract
In order to study the functions and activities of chromatin remodeling enzymes in vitro, it is necessary to be able to reconstitute nucleosomes on DNA templates. In this chapter, we describe procedures for purification of histones from E. coli, formation of octamers, and reconstitution of nucleosomes that can be further modified by chromatin modifiers. In addition, we describe methods to purify nucleosomes from human cells. Finally, we also describe assays to test binding and remodeling activities of chromatin remodelers.
Publication Title, e.g., Journal
Methods in Molecular Biology
Volume
1528
Citation/Publisher Attribution
Dutta, Arnob, and Jerry L. Workman. "In vitro assembly of nucleosomes for binding/remodeling assays." Methods in Molecular Biology 1528, (2017): 1-17. doi: 10.1007/978-1-4939-6630-1_1.