Butanol production by Clostridium pasteurianum ATCC™ 6013 using biodiesel-derived crude glycerol: Microbial response to environmental stress

Document Type

Conference Proceeding

Date of Original Version



During the production of biodiesel, crude glycerol is produced as a byproduct at 10% (w/w). The crude glycerol has to be disposed, as the undisposed glycerol affects the sustainability of the biodiesel plants. C. pasteurianum has the inherent potential to grow on glycerol and produce 1,3-propanediol and butanol as the major products. Butanol, at higher concentrations, affects the growth and metabolism of the cells by directly affecting the fluidity of the cell membrane. In this study we present results regarding the stability and the degree of unsaturation of the cell membrane of cells exposed to different concentrations of exogenous butanol as well as cell producing various concentrations of butanol. We will discuss the results from the impact of the impurities in crude glycerol, such as methanol, salts and fatty acids, on the growth and metabolism of C. pasteurianum.

Publication Title, e.g., Journal

ACS National Meeting Book of Abstracts

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