Title

Kinetics of microbial oxidation of n‐heptane. I. Characterization of the process

Document Type

Article

Date of Original Version

1-1-1969

Abstract

Utilization of n‐heptane by a Pseudomonad was studied in pilot‐size butch cultures. Optimal pH and temperature were determined by a factorial design and a medium based upon mineral uptake rates was formulated. High cell yields were obtained by volatilizing heptane in the incoming air and thereby achieving good hydrocarbon dispersion. Hydrocarbon carried by effluent gases was recovered and recycled. In cultures where pH is not controlled, decrease in the electrolytic conductivity of the medium was found to be indicative of viable cells and was used in monitoring bacterial propagation. If not checked, increase in salinity in pH controlled cultures was found to affect cell production negatively. Viscosity changes were not very significant. Heptane to aqueous medium ratio was found to affect oxygen supply to the system due to higher dissolved oxygen concentrations associated with hydrocarbons. Copyright © 1969 John Wiley & Sons, Inc.

Publication Title

Biotechnology and Bioengineering

Volume

11

Issue

3

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