Date of Original Version
Escherichia coli F-18, isolated from the feces of a healthy human in 1977, is an excellent colonizer of the streptomycin-treated mouse large intestine and displays normal motility and chemotaxis ability. A chemotaxis-defective derivative of E. coli F-18, E, coli F-18 CheA-, and a nonflagellated derivative, E. coli F-18 Fla-, were constructed. These strains were found to colonize the streptomycin-treated mouse large intestine as well as E. coli F-18 when mice were fed both E. coli F-18 and either the CheA- or Fla- derivative at high levels (1010 CFU of each strain per mouse) or low levels (104 CFU of each strain per mouse). Furthermore, E. coli F-18 lost motility and chemotaxis ability when grown in colonic or cecal mucus in vitro despite retaining the ability to synthesize flagella. Thus, it appears that neither motility nor chemotaxis plays a role in the ability of E. coli F-18 to colonize because this strain becomes functionally nonmotile upon growth in the streptomycin-treated mouse large intestine.
McCormick, B. A., Laux, D. C., & Cohen, P. S. (1990). Neither Motility nor Chemotaxis Plays a Role in the Ability of Escherichia coli F-18 To Colonize the Streptomycin-Treated Mouse Large Intestine. Infect. Immun., 58(9), 29557-2961. Retrieved from https://iai.asm.org/content/58/9/2957.
Available at: https://iai.asm.org/content/58/9/2957