Date of Original Version
Arginine was the only amino acid used by Clostridium difficile that permitted cytotoxin synthesis in a peptone-based medium. Synthesis of cytotoxin was delayed when glucose was used as the substrate. Addition of rifampin or puromycin to cultures prior to release of cytotoxin inhibited the release of cytotoxin, suggesting that a protein essential for cytotoxin release is synthesized after cytotoxin is synthesized.
Osgood, D. P., Wood, N. P., & Sperry, J. F. (1993). Nutritional aspects of Cytotoxin producation by Clostridium difficile. Appl. Env. Micriobol., 59(12), 3985-3988. Retrieved from https://aem.asm.org/content/59/12/3985.
Available at: https://aem.asm.org/content/59/12/3985