Pomegranate (Punica granatum) phenolics ameliorate hydrogen peroxide-induced oxidative stress and cytotoxicity in human keratinocytes
Date of Original Version
Pomegranate phenolics have been reported to exert skin beneficial effects but their mechanisms of action remain unclear. Herein, we investigated a standardized commercial pomegranate extract (PE; Pomella®) and its phenolics including punicalagin (PA), ellagic acid (EA), and urolithin A (UA) for their protective effects against hydrogen peroxide (H 2 O 2 )-induced oxidative stress and cytotoxicity in human keratinocyte HaCaT cells. PE, PA, and EA reduced the production of H 2 O 2 -induced ROS in HaCaT cells by 1.03-, 1.37-, and 2.67-fold, respectively. PE, PA, and UA increased the viability of H 2 O 2 -stimulated HaCaT cells by 89.9, 94.9, and 90.0%, respectively. PE, PA, and UA reduced apoptotic cell populations by 3.39, 7.11, and 8.26%, respectively. In addition, PE, PA and UA decreased H 2 O 2 -stimulated caspase-3 level by 2.31-, 2.06-, and 2.68-fold, respectively. The ameliorative effects of this PE and its phenolics against the H 2 O 2 -induced oxidative stress and cytotoxicity in keratinocytes support their utilization as natural cosmeceuticals for skin health.
Publication Title, e.g., Journal
Journal of Functional Foods
Liu, Chang, Hao Guo, Nicholas A. DaSilva, Dongli Li, Kun Zhang, Yinsheng Wan, Xing Hua Gao, Hong Duo Chen, Navindra P. Seeram, and Hang Ma. "Pomegranate (Punica granatum) phenolics ameliorate hydrogen peroxide-induced oxidative stress and cytotoxicity in human keratinocytes." Journal of Functional Foods 54, (2019): 559-567. doi: 10.1016/j.jff.2019.02.015.