Sikokianin A from Wikstroemia indica protects PC12 cells against OGD/R-induced injury via inhibiting oxidative stress and activating Nrf2
Date of Original Version
Ischemic cerebral stroke is a severe cause of human death and disability. Natural products play an important role in the discovery of novel therapy for cerebral ischemia. Herein, we investigate the neuroprotective effects of sikokianin A identifiedfrom Wikstroemia indica using PC12 cell exposed to OGD/R. The results revealed sikokianin A can improve the poor viability and release of intracellular LDH in PC12 cells induced by OGD/R. Further studies have demonstrated the increased ROS and MDA together with reduced SOD activity were attenuated by sikokianin A. Meanwhile, decreased mitochondrial membrane potential, activated Caspase-3, down-regulated Bcl-2 and up-regulated Bax were reversed. These results indicate the protective effects of sikokianin A are associated with inhibiting oxidative stress and apoptosis resulting from OGD/R. Additionally, sikokianin A can activate Nrf2 and downstream HO-1 in PC12 cells treated by OGD/R, which implied Nrf2/HO-1 signaling pathway was involved in the protective effects of sikokianin A.
Natural Product Research
Yao, Huankai, Wenting Zhang, Huiling Wu, Ming Yang, Pengxiang Wei, Hang Ma, Jingyu Duan, Chunping Zhang, and Yan Li. "Sikokianin A from Wikstroemia indica protects PC12 cells against OGD/R-induced injury via inhibiting oxidative stress and activating Nrf2." Natural Product Research 33, 23 (2019): 3450-3453. doi:10.1080/14786419.2018.1480019.