Title
Tea polyphenols induced apoptosis of breast cancer cells by suppressing the expression of Survivin
Document Type
Article
Date of Original Version
3-20-2014
Department
Biomedical and Pharmaceutical Sciences
Abstract
To study the mechanism of tea polyphenols (TP)-induced apoptosis of breast cancer cells. Proliferation of MCF-7 and SK-BR-3 cells was evaluated by MTT assays. Cellular ultrastructure was examined by electron microscopy. Apoptosis was detected by TUNEL. PCNA, Cyclin D1, Cyclin E and Survivin expression was measured by Western blot. Cell proliferation was significantly inhibited by TP. Spindle and round cells were loosely distributed with increased particles after TP treatment. Increased cell size, frequent nuclear atypia and a collapse of apoptosis were observed. The nucleus was pushed towards one side, while the cytoplasm was rich in free ribosome. The membrane of mitochondria was thickening, and the cell apoptotic body was observed. TP treated cells experienced significantly enhanced apoptosis compared with 5-Fu treated or control groups. The expression of survivin was downregulated by TP. To conclude, TP can inhibit cell growth and induce apoptosis through downregulating the expression of survivin in breast cancer.
Citation/Publisher Attribution
Chen, X., Li, Y., Lin, Q., Wang, Y., Sun, H., Wang, J., Cui, G.,...Dong, X. (2014). Tea polyphenols induced apoptosis of breast cancer cells by suppressing the expression of Survivin. Scientific Reports. 4: 4416. doi: 10.1038/srep04416
Available at: http://dx.doi.org/10.1038/srep04416
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.