Date of Award

2014

Degree Type

Thesis

Degree Name

Master of Science in Biological and Environmental Sciences (MSBES)

Specialization

Cell and Molecular Biology (CMB)

Department

Cell & Molecular Biology

First Advisor

Niall Howlett

Abstract

Fanconi Anemia (FA) is a rare genetic disease caused by biallelic mutations in one of sixteen genes involved in the FA-BRCA DNA damage repair pathway. The proteins encoded by these genes function cooperatively in a common pathway which resolves lesions caused by interstrand crosslinks (ICLs). A critical step in this pathway is the monoubiquitination and chromatin targeting of FANCD2 and FANCI. The mechanism by which these proteins are targeted to chromatin is not understood. FANCD2 is known to interact with several downstream proteins while associated with chromatin. Finding new FANCD2 interacting proteins is critical to understanding how FANCD2 functions and how it is regulated within the cell. I have identified several candidate interacting proteins by immunoprecipitations (IPs) coupled with mass spectrometry. Candidates include transcription factors, chromatin remodeling complex components and proteins involved in chromosome maintenance and stability. These interactions are being validated and functionally characterized using a variety of techniques.

Share

COinS