Date of Award

1998

Degree Type

Thesis

Degree Name

Master of Science in Microbiology

Department

Biochemistry, Microbiology, and Molecular Genetics

First Advisor

Paul S. Cohen

Abstract

A DNA fragment containing the defective virulence gene(s) in the live oral vaccine candidate Salmonella typhimurium SR-11 Fad was isolated. Genomic DNA fragments from the vaccine candidate, constructed by Tn10d::cam transposon mutagenesis, were probed with the 1.4 Kbp chloramphenicol resistance gene contained in Tn10d::cam and analyzed by Southern hybridization. Two of the fragments, an 8.0 Kbp Hind III fragment and a 4.5 Kbp Pst I fragment, were isolated, cloned into E. coli HB 101, and mapped. The Pst I fragment, containing approximately 2.9 Kbp of flanking genomic DNA, was subcloned into the sequencing vector pBluescript II SK + (Stratagene, La Jolla, CA) and mapped.

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