Functional silane based co-polymers for biofunctionalization studies, chemical sensing and separations
The focus of the research reported in this dissertation has focused on two areas: (1) Synthesis of silane-containing polymers and (2) the application of these polymers to surface modification of substrates such as PDMS (for microfluidic applications), glass, quartz, silicon wafers and various nanoparticles (magnetic iron oxide, silica) for use in chemical and biological sensing and separation applications as well as in biofunctionalization of magnetic nanoparticles. ^ From the library of silane containing co- and terpolymer prepared with various susbtituted maleimides are characterized by FTIR, UV-Visible, 1H- and 13C-NMR, TGA and DSC. These structures are being explored for their use in chemical sensing and separations of explosive and amines and other species. A facile biofunctionalization of magnetic and silica nanoparticles is also describe in this dissertation. The ability to biofunctionalize these materials allows for their potential application in the biomedical field where they can be used in detection and in targeted drug delivery. ^ Also described herein is the functionalization of microfluidics systems for bioanalysis. Surface modification with silica nanoparticles would be a promising technique to provide an excellent interface on the conventional polymer surface. A sensitive biofunctionalization is carried in microfluidic chips. The antigens attached to the surface modified silanes via urea linkage and successful binding of antigens and antibodies is observed and can preserve the bioactivity of the antigens and antibodies and resist non specific adsorption. This study can be extended for a high-throughput system for bio-marker proteins. ^ Finally, we demonstrated the applicability of these polymers for sensing amines and nitro compounds. Substitutions on the phenyl ring help in tuning the sensitivity of these polymers to various amines including very weak aromatic amines. Increase in the intensity of fluorescence and a very noticeable change in color of the polymer solutions in presence of amines helps in their detection. This work represents our ability to design, control and utilize novel materials for various applications. ^
"Functional silane based co-polymers for biofunctionalization studies, chemical sensing and separations"
Dissertations and Master's Theses (Campus Access).