Fish landmarks for chromosome 11 of Bombyx mori: An in situ analysis
Abstract
Fluorescence in situ hybridization (FISH) has been used to simultaneously identify the positions of the ribosomal DNA and the Sericin genes on chromosome 11 of Bombyx mori. The ribosomal DNA genes were detected using biotin labeled plasmid pBmR 161 and avidin-Texas Red and the Sericin 2 gene was detected using digoxigenin labeled plasmid 2002 and anti-digoxigenin-fluorescein. The position of the Sericin 1 gene was identified using the ribosomal DNA genes as a chromosomal landmark. Sericin 1 was detected using digoxigenin labeled bacterial artificial chromosome 9HJD and anti-digoxigenin-fluorescein. The chromosomal spreads were counterstained with diamindino-phenyl-indole (DAPI). The signals from the sericin genes consistently co-localized with the ribosomal DNA signals and existed as a single pair of hybridization signals on pachytene chromosomes from 5th instar male silkworms. ^
Subject Area
Biology, Genetics|Biology, Zoology
Recommended Citation
Charles Eugene McGovern,
"Fish landmarks for chromosome 11 of Bombyx mori: An in situ analysis"
(2002).
Dissertations and Master's Theses (Campus Access).
Paper AAI3053113.
http://digitalcommons.uri.edu/dissertations/AAI3053113